Evaluation by in vivo respiration enrolled of lung toxicity in rats kept in coal mine ambience: effect of antioxidant therapy
Seyfullah Oktay Arslan
Department of Pharmacology
Duzce University, Medical Faculty, Turkey
Abstract:
Occupational exposure to coal dust causes pneumoconiosis and other lung diseases. Reactive oxygen species (ROS) have been implicated in the pathogenesis of coal dust-induced lung toxicity.
In this study, we investigated the respiration in rats lung toxicity exposed to coal dust in mine ambience and hydroxyproline (HP) levels in lung tissues. In addition, we also investigated the attenuating effects of erdosteine as antioxidant therapy. Rats were exposed to mine ambience for four week, and then they were breathed in the clean air for four week. The respiratory functions of rats were recorded once a week for eight week, as in vivo. At the end of the processes, tissue levels of HP, malondialdehyde (MDA) and NO, as well as the activities of glutathione peroxidase and myeloperoxidase (MPO) were evaluated in the lung tissues or BALFs of rats.
We observed to be beginning the respiratory abnormalities in animals to expose to coal dust in second week. The end of fourth week, there were the increase of respiratory frequency and along with the decrease of respiratory depth. The respiratory failures were not improved in clean ambience, moreover apnea were appearance in the end of six week (the second week of clean air). The percent 28 of animals were died. Erdosteine administer to rats were not abolished pulmonary toxicity, however could able to hold to toxicity, and also there were not dies in rats administered to erdostein. Exposure to coal dust resulted in a significant increase in the oxidant parameters (MDA and NO levels) and HP levels, as compared to the controls. A decrease in activities of antioxidant enzymes, and an increase in MPO activity were found in the study group, compared to the controls. Increased NO levels of lung were found in the study groups, that were significantly reduced by erdosteine.
Our experimental studies provide evidence that supports the hypothesis for ROS induced coal workers' pneumoconiosis. Reoxygenation can both not to get it together to pulmonary toxicity and more and more exaggerate. On this account reoxygenation should be by steps in mine workers. In addition the processes of antioxidant therapy may be beneficial in the coal dust-induced lung toxicity of coal workers.
Keywords: respiratory functions, antioxidant therapy, reoxygenation, oxidative stress, coal mine ambience, rats
